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Index > Protein center > Igf2(Gene name) > Rat
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  • Igf2 (Gene name),
  • Insulin-like growth factor II (Protein name ),  IGF2_RAT from NCBI database.
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  • General Annotation
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  • Antigen Annotation
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  • 3D
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  • Predicted Eptitope
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  • Vaild Sequence
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  • Related Databases
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  • Gene name:
    Igf2(Igf-2);
    Protein name:
    Insulin-like growth factor II(IGF-II);
    Alternative:
    Multiplication-stimulating polypeptide;Multiplication-stimulating activity(MSA);
    Organism:
    Rat (Rattus norvegicus). 
    General Annotation
    Sub Unit:
    N/A
    Function:
    Preptin undergoes glucose-mediated co-secretion with insulin, and acts as physiological amplifier of glucose-mediated insulin secretion. Exhibits osteogenic properties by increasing osteoblast mitogenic activity through phosphoactivation of MAPK1 and MAPK3.
    Subcellular Location:
    Secreted
    Protein Attributes:
    Sequence length:
    180
    Sequence:
    50:
    MGIPVGKSML | VLLISLAFAL | CCIAAYRPSE | TLCGGELVDT | LQFVCSDRGF | 
    100:
    YFSRPSSRAN | RRSRGIVEEC | CFRSCDLALL | ETYCATPAKS | ERDVSTSQAV | 
    150:
    LPDDFPRYPV | GKFFKFDTWR | QSAGRLRRGL | PALLRARRGR | MLAKELEAFR | 
    180:
    EAKRHRPLIV | LPPKDPAHGG | ASSEMSSNHQ | 
    3D Structure:
    N/A
    Predicted Eptitope:
    Please Sign in.
    EIAab Sequence  Vaild Sequence:
    Please Sign in.
    Related Databases
    UniGene:
    KEGG:
    String:
    SMR:
    Pfam:
    Uniprot:
     
    FOR
    ELISA Kit for Rat IGF-II
    Cat.:
    E0051r
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    96T
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    ELISA Kit for Rat IGF-II
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    E0051p
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    96T
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    ELISA Kit for Rat IGF-II
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    E0051m
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    96T
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    ELISA Kit for Rat IGF-II
    Cat.:
    E0051h
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    96T
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    ELISA Kit for Rat IGF-II
    Cat.:
    E0051c
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    96T
    ELISA Kit for Rat IGF-II
    Cat.:
    E0051b
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    96T
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    CLIA Kit for Rat IGF-II
    Cat.:
    U0051b
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    MSDS:
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    Packing:
    96T
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    CLIA Kit for Rat IGF-II
    Cat.:
    U0051c
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    MSDS:
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    Packing:
    96T
    CLIA Kit for Rat IGF-II
    Cat.:
    U0051h
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    Please sign in first.
    MSDS:
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    Packing:
    96T
    Range:
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    CLIA Kit for Rat IGF-II
    Cat.:
    U0051m
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    MSDS:
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    Packing:
    96T
    Range:
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    CLIA Kit for Rat IGF-II
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    U0051p
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    MSDS:
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    Packing:
    96T
    Range:
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    CLIA Kit for Rat IGF-II
    Cat.:
    U0051r
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    MSDS:
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    Packing:
    96T
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    Polyclonal Antibody for Rat IGF-II
    Cat.:
    P0051Rb-m
    Price:
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    Packing:
    40ug/0.2ml
    Polyclonal Antibody for Rat IGF-II
    Polyclonal Antibody for Rat IGF-II
    Cat.:
    P0051Rb-r
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    Packing:
    40ug/0.2ml
    Polyclonal Antibody for Rat IGF-II
    Polyclonal Antibody for Rat IGF-II
    Cat.:
    P0051Rb-h
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    Packing:
    40ug/0.2ml
    Polyclonal Antibody for Rat IGF-II
    Monoclonal Antibody for Rat IGF-II
    Monoclonal Antibody for Rat IGF-II
    Monoclonal Antibody for Rat IGF-II
    Monoclonal Antibody for Rat IGF-II
    Monoclonal Antibody for Rat IGF-II
    Monoclonal Antibody for Rat IGF-II
    Protein for Rat IGF-II
    Protein for Rat IGF-II
    Protein for Rat IGF-II
    Protein for Rat IGF-II
    Protein for Rat IGF-II
    Protein for Rat IGF-II

    R&D Technical Data
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    s

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    Precision
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    Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

    Intra-Assay CV: ≤3.8%

    Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.

    Inter-Assay CV: ≤6.7%

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    Recovery
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    Recovery was determined by spiking various levels of IGF2 into serum and plasma .

    Sample Type

    Average(%)

    Recovery Range(%)

    Serum

    91

    87-95

    Plasma

    92

    90-97

     

     

     

     

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    Linearity
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    The linearity of the kit was assayed by testing samples spiked with appropriate concentration of IGF2 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.  

     

    Sample

    1:2

    1:4

    1:8

    1:16

    serum(n=5)

    90-96%

    85-92%

    87-98%

    91-97%

    EDTA plasma(n=5)

    93-95%

    88-96%

    95-100%

    86-93%

    heparin plasma(n=5)

    88-94%

     

    91-98%

    100-106%

    80-87

     

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    References
    1. 1.
      "Insulin-like growth factor II precursor gene organization in relation to insulin gene family."
      Dull T.J. , Gray A. , Hayflick J.S. , Ullrich A.
      Nature310:777-781(1984) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE
      strain: BRL-3A.
    2. 2.
      "Developmental and tissue-specific expression of a family of transcripts related to rat insulin-like growth factor II mRNA."
      Soares M.B. , Ishii D.N. , Efstratiadis A.
      Nucleic Acids Res.13:1119-1134(1985) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA]
      strain: Buffalo.
    3. 3.
      "Rat insulin-like growth factor II gene. A single gene with two promoters expressing a multitranscript family."
      Soares M.B. , Turken A. , Ishii D.N. , Mills L. , Episkopou V. , Cotter S. , Zeitlin S. , Efstratiadis A.
      J. Mol. Biol.192:737-752(1986) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE
    4. 4.
      "Structure and expression of the rat insulin-like growth factor II (rIGF-II) gene. rIGF-II RNAs are transcribed from two promoters."
      Frunzio R. , Chiariotti L. , Brown A.L. , Graham D.E. , Rechler M.M. , Bruni C.B.
      J. Biol. Chem.261:17138-17149(1986) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]
    5. 5.
      "Transcriptional deviation of the rat insulin-like growth factor II gene initiated at three alternative leader-exons between neonatal tissues and ascites hepatomas."
      Ueno T. , Takahashi K. , Matsuguchi T. , Endo H. , Yamamoto M.
      Biochim. Biophys. Acta950:411-419(1988) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE
    6. 6.
      "Isolation of a cDNA clone encoding rat insulin-like growth factor-II precursor."
      Whitfield H.J. , Bruni C.B. , Frunzio R. , Terrell J.E. , Nissley S.P. , Rechler M.M.
      Nature312:277-280(1984) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE OF 62-180
    7. 7.
      "Structure of the rat insulin-like growth factor II transcriptional unit: heterogeneous transcripts are generated from two promoters by use of multiple polyadenylation sites and differential ribonucleic acid splicing."
      Chiariotti L. , Brown A.L. , Frunzio R. , Clemmons D.R. , Rechler M.M. , Bruni C.B.
      Mol. Endocrinol.2:1115-1126(1988) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE OF 103-180
    8. 8.
      "Purification and primary structure of a polypeptide with multiplication-stimulating activity from rat liver cell cultures. Homology with human insulin-like growth factor II."
      Marquardt H. , Todaro G.J. , Henderson L.E. , Oroszlan S.
      J. Biol. Chem.256:6859-6865(1981) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: PROTEIN SEQUENCE OF 25-91
    9. 9.
      "Preptin, another peptide product of the pancreatic beta-cell, is osteogenic in vitro and in vivo."
      Cornish J. , Callon K.E. , Bava U. , Watson M. , Xu X. , Lin J.M. , Chan V.A. , Grey A.B. , Naot D. , Buchanan C.M. , Cooper G.J. , Reid I.R.
      Am. J. Physiol.292:E117-E122(2007) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: OSTEOGENIC FUNCTION OF PREPTIN
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